This mixture is then incubated at fifty five°C for one hour. Then the sample is phenol-extracted after with phenol/chloroform/isoamyl Alcoholic beverages Remedy and centrifuged. The aqueous layer is eradicated to some fresh new microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a second time. Following the pellet https://www.pinterest.com/pin/993958580252568763/