Typically this stress is 0.25 inches of h2o column, but might be elevated or lowered as the method requires. Isolators need to be periodically leak tested to make sure their integrity and prevent escape from the decontamination agent. Leak testing can be done by various techniques such as tension https://tailinscitech.wordpress.com/2026/01/16/applications-and-benefits-of-tailin-containment-isolator-for-handling-high-potency-materials/